iDiagnostics - the handheld future of precision medicine; Single Molecule Detection, Single Cell Analysis, Cell Membrane Studies, TIRF microscopy, TIRF accessories for fluorometers, TIRF biosensor instruments, Real-time TIRF microarrays, and more>>> visit www.tirf-labs.com
Molecular iDiagnostics for Home Use
by TIRF Labs
Literature (Selected publications of our customers and our own data generated by TIRF Labs' products)
1. Asanov A. “TIRF-EC Biosensors Massively parallel DNA and Protein Microarrays for Accurate and Rapid Detection of Pathogens.” Biodetection Technologies 2007. Knowledge Press, Brookline, MA. 4th Edition, 2007, Chapter 17, p. 373-396, isbn # 1-59430-126-3.
2. Asanov A. “TIRF-EC Biosensors with Reagentless Bioassays for Rapid and Accurate Detection of Pathogens.” Biodetection Technologies 2008. Knowledge Press, Brookline, MA. 5th Edition, 2008, Chapter 8, p. 155-190, isbn # 1-59430-137-9.
3. Asanov A., Wilson W., and Oldham P. “Regenerable biosensor using total internal reflection fluorescence with electrochemical control”. US Patent 6,511,854. Publication Date: 2003-01-28. (Active patent.)
4. Asanov A., Zepeda A., Vaca L. “Combined DNA and Protein Microarrays Based on Total Internal Reflection Fluorescence.” Sensors, 2012, 12, 1800.
5. Patents pending 2016-2017.
6. Willoughby D., Everett K., Halls M., Pacheco J., Skroblin P., Vaca L., Klussmann E., and Cooper D. “Direct Binding Between Orai1 and AC8 Mediates Dynamic Interplay Between Ca2+ and cAMP Signaling.” Science Signaling, 2012, 5 (219), 29.
7. Sampieri A., Zepeda A., Asanov A., and Vaca L. “Visualizing the store operated channel complex assembly in real time: Identification of SERCA2 as a new member.” Cell Calcium, 2009, 45, 439.
8. Asanov A., Zepeda A., and Vaca L. “A novel form of Total Internal Reflection Fluorescence Microscopy (LG-TIRFM) reveals different and independent lipid raft domains in living cells.“ Biochim. Biophys. Acta, 2010, 1801, 147.
9. Asanov A, Sherry R, Sampieri A, and Vaca L. “A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at endoplasmic reticulum plasma membrane junctions”. Cell Calcium, 2013, 54, 246– 256
10. Luz-Madrigal A, Asanov A, Camacho-Zarco AR, Sampieri A, and Vaca L. “A Cholesterol Recognition Amino Acid Consensus Domain in GP64 Fusion Protein Facilitates Anchoring of Baculovirus to Mammalian Cells”. Journal of Virology, 2013, 87 (2), 11894–11907.
11. Additional publications of our customers with data generated by our TIRF and TIRF-EC products are available at the URL: www.tirf-labs.com
iDiagnostics Application Development Kit
and uTIRF Biodetection Station
The early detection of cancer, cardiovascular and other diseases, preventing pandemics, and other healthcare tasks require a highly sensitive, accurate, rapid, and yet affordable molecular diagnostics. Eleven years of research at TIRF Labs have resulted in the development of such a technology: a handheld platform, named iDiagnostics, which is a thousand times more accurate than traditional methods, rapid and robust, and yet inexpensive.
The goal of TIRF Labs is to provide iDiagnostics to every family on the globe. This goal cannot be achieved by a single company; we envision a collective global effort from the diagnostics community using the Open Innovation Business Model, which will stimulate a flux of intellectual property (IP) in and out of the iDiagnostics project. TIRF Labs will be delighted to license the IP of other research groups and will offer the opportunity to license our own IP, in certain cases free of charge.
To facilitate all of the stages of this challenging project, from biomarker discovery, to clinical testing, TIRF Labs offers the uTIRF biodetection station and the Application Development Kit (ADK). Figs. 1 and 2 show the optical scheme and photos of uTIRF, the lightguide-based TIRF accessory, and manual microarrayer. Fig. 3 depicts the iDiagnostics handheld cradle, and reusable cartridges designed for the development stage. These products are available to all members of the diagnostic community, including academic, industrial, and government research groups. The NIH, FDA and CDC will be involved in the coordination of the collective efforts.
Two main products - the uTIRF biodetection station and the Application Development Kit (ADK) - create the foundation for the collective project. The uTIRF is a multifunctional biodetection station, which is sensitive down to single molecules. It includes lightguide-, prism-, and objective-based TIRF, a low light CMOS camera, three-color illuminator, and 1X, 4X, and 60X objectives. The iDiagnostics ADK consists of the iDiagnostics handheld cradle, cartridge blanks, manual microarrayer, and reagents kit.
TIRF Labs has already received numerous requests from research groups worldwide to supply these products. In the middle of 2018 we plan to start supplying these products along with experimental protocols. Several groups have already joined our project. We have incorporated their feedback and will incorporate future comments from our future customers in the next releases of the uTIRF and ADKs. We will supply advanced hardware, software, reagents, development tools, protocols, videos, and other support to accommodate the requirements for different diagnostic applications.
The arrays of bioassays printed at the surface of iDiagnostics slides include assays for detecting protein, nucleic acid, metabolite biomarkers. The arrays contain internal controls to ensure reliability and calibration. The cartridge is equipped with a 20-microliters flow cell, which encompasses the microarray. iDiagnostics requires no or minimum sample preparation. Whole blood can be analyzed after the addition of an anticoagulant. Simple sample preparation module is included in the cartridge; additional space is reserved for more complex sample preparation modules as will be required for applications developed in the future. For more information see iDiagnostics brochure, visit i-diagnostics.net and tirf-labs.com websites, and contact us via email: email@example.com
iDiagnostics is a novel molecular diagnostics platform based on real-time TIRF microarrays, which is supersensitive, accurate, and rapid, yet affordable for home use. If you are developing fluorescence, electro-chemi-luminescence (ECL), or bioluminescence bioassays for diagnosing or prognosing, we invite you to collaborate. We will help you to interface your assays with iDiagnostics platform and will be delighted to license your technology. Ask about uTIRF and iDiagnostics Application Development Kit (ADK). Visit i-Diagnostics.net website.
iDiagnostics features a novel type of real-time microarray that simultaneously detects protein, nucleic acid, and metabolite biomarkers. It requires no or minimal sample preparation and is capable of detecting from a single to several thousands of molecular markers in a 50-microliter sample of biological fluids, including whole blood. High sensitivity and the broad dynamic range of iDiagnostics covers the entire spectrum of clinically significant concentrations. Limit of Detection (LOD) for micro-RNA is ~10-18M. For proteins and metabolites LOD depends on the assay; for certain antibody-based assays LOD is ~10-15M.
Classical TIRF microarrays operate with small, sub-monolayer amounts of antibodies and DNA probes immobilized on the surface; fluorescence signal is small, a low light photodetector, e.g. EMCCD camera is necessary. In the patent-pending iDiagnostics technology the signal of TIRF arrays is enhanced by 3D encapsulation, which captures the excitation light and becomes an integral part of the lightguide. 3D encapsulation allows for using larger amounts of antibodies per unit area of bioassay spot. The signal from such arrays is a thousand-fold greater than that in classical arrays. CCD cameras of cellphones are sensitive enough to detect the signal. iDiagnostics is ideal platform for interfacing antibody-based bioassays for detecting proteins and molecular beacon assays for measuring nucleic acids. Visit tirf-labs.com website and contact us by email: firstname.lastname@example.org for more information.
Molecular iDiagnostics (iTIRF microarray)