iDiagnostics - the handheld future of precision medicine; Single Molecule Detection, Single Cell Analysis, Cell Membrane Studies, TIRF microscopy, TIRF accessories for fluorometers, TIRF biosensor instruments, Real-time TIRF microarrays, and more>>> visit www.tirf-labs.com
Molecular iDiagnostics for Home Use
by TIRF Labs
How iDiagnostics Was Born
iDiagnostics is the result of an 11-year effort by TIRF Labs’ group of scientists and engineers. We have received $4.3 million in grants from the U.S. government and have developed a super-sensitive technology - 3D-enhanced TIRF microarrays. As mentioned above, TIRF is an exceptionally sensitive method capable of detecting biological particles down to single molecules. Fig. 6 shows our progress in the development of TIRF array biosensors, which spanned from large bench-top instruments created in 2005, through portable sensors produced in 2009, to small handheld devices built in 2011. In 2013, we discovered that silk fibroin enhances the fluorescence of TIRF microarrays so that we can use a cellphone camera instead of expensive cameras. This patent-pending discovery has been incorporated into iDiagnostics. In Phase 1 of the project iDiagnostics will migrate back to the handheld configuration, in which the cradle will be equipped with its own low light camera and a microcomputer. This turn will add little to the cost of the cradle, and will allow for avoiding several issues related to the use of cellphone software and to alignment of different cellphones with the cartridge.
Fig. 6. Downsizing TIRF microarray biosensors.